Find regional support. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Version 7. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. style. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. . The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Supported Windows Operating System: See release notes. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. SpectraMax® i3x. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. A: percent GFP-positive cells calculated using the classification feature in the software. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 312 gain calibration failed. Read modes. Pick the best speed, sensitivity, and price for your needs. Monday to Friday 8am - 5pm GMT. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Data analysis. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. . Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Unlimited breadth of. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Table 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Figure 2-23: Bottle holder To install the bottle holder: 1. . The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. china@moldev. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. SpectraMax i3x Register or Sign In. Contact Us Phone: +1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Description 1. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. compound concentration, and an IC 50 value of 21. 3) At the bottom of the strip, click on "Show. 22 μ m fi ltration. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 1 and above: Compatible with only with 64-bit OS. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. 1. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Description 1. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. Find our technical support information along with contact details. Example: [Setup]. 7. SpectraMax i3x, onboard luminescence 121. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 800. The e ect of ASC-exosomes on the melanin levels inThe 70% methanolic extract of steamed root (100 µL) was added to 1 mL of diethylene glycol and 10 µL of sodium hydroxide (NaOH). Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. . In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. 306 plate air cal fail,low light. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. . EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. QuickSync. 2 x 10-14. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Not compatible with Home Edition. SpectraMax i3x Register or Sign In. techsupport. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Note: Additional readers with similar performance are listed. As. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. View the Molecular Devices website. Find regional support. SpectraMax i3, i3x, and iD5 readers. 2. 5 Figures from 202 Publications Thermo Fisher Scientific. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. 4. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. EUROPE. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The plate was shaken and incubated in the dark for 10 minutes. infoboxeu@moldev. . Version 7. 0001 0. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax® ABS and ABS Plus reader support; ü. Danaher Inc spectramax i3x. EMax Plus Microplate Reader. to View Figures. 0´ 104 8. SpectraMax Quant dsDNA Assay Kits. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Description. . 302 low ref light. Determination of anti-inflammatory. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Explore a wealth of applications with the future-ready SpectraMax i3x. Plaque Reduction Neutralization Test (PRNT) SARS-CoV-2 neutralization was assessed as previously described . The plate was shaken and incubated in the dark for 10 minutes. D 1 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The dynamic range of the assay is about 3. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. 1 Microplate Data Acquisition & Analysis Software for Windows. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. eu@moldev. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. 315 can't find zero order. SpectraMax i3x readers. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. . The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. 99. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Summary of Contents for Molecular Devices SpectraMax M2. 5 µL anti-cAMP-Cryptate. The SpectraMax® i3x Multi-Mode Microplate Reader evolves with your future needs and offers an unlimited breadth of application possibilities. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 305 reference level saturation. The information below applies to a standard 96 well plate, set to a linear shake mode. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Spectranet customer portal. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Shake intensity. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 1. The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Replicate values were averaged and imported into Design-Expert 8. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. 1. Negative samples contained only MycoAlert Assay Buffer. 312 gain calibration failed. i3. Multimode Readers Comparison Chart. SoftMax Pro. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. i3x single use: $35: $52. Contact Local Team. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Download PDF. This process should take about 3 - 5 minutes. Methods. Curves were forced through x,y = 0. Download SoftMax® Pro 7. . Bioz Stars score: 98/100, based on 1 PubMed citations. Table 1. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Molecular Devices Software. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Data Analysis. Background subtraction was not necessary. Nucleic Acid and Protein Quantitation. com. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Citation 2002). Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Prepare your i3/i3x instrument for shipment. com. 1. Failed to load PDF file. D 1 1. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Eliminate time-dependent substrate addition steps. More Product Information. My proficiency in techniques such as •PBMC isolation, counting and. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). It allows you to explore cellular pathways and protein activation and expression in one system. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. We would like to show you a description here but the site won’t allow us. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. 5577 Web: Email: info@moldev. . SpectraMax® Microplate Readers and SoftMax® Pro Software. Calcium. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 7 Figures from 1126 Publications. The treatments were. ATP (“flash” luminescence using . 6% for the on-board optics (monochromators). Modularity does provide some measure of forward vision into upcoming or future experimental needs. 2. Supported Windows Operating System: See release notes. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax® i3x. i3x Specifications. 012 fmol 0. SpectraMax® L reader support; ü. 3. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Simplified workflow: Add cellular imaging assays in a small footprint. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The chamber. Multimode Reader Comparison Chart. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Rutin was used to generate standard curves for quantification. Figure 1. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. Both readers feature an 8-channel read head for fast reads and. 313 reference gain check fail. It can be upgraded with. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Actual shake speed is based on the microplate format. Request a Quote for This Setup. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. HTRF-compatible SpectraMax Multi-Mode Readers. Product Details. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Both Microplate Optimization and Read Height Adjustment were. 58% for the detection module and 2. Serial number 33 900 01-3387. The calculated LOQ values were 0. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Nuclear Receptor. 0´ 104 6. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. . . The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Features. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. Find regional support. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. 12911151) 0. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Learn more about its features, specifications, and applications in this user guide. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Our absorbance plate readers feature our PathCheck Sensor. Published Dec 16, 2020 | Updated Oct 10, 2023. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. SeeSpectraMax MiniMaxImagingCytometeronpage85. 0 mm hex key. Background Parallax,Columns 2. 1 software Download page. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 2in. Example: [Drawer]. . The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. D 1 1. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. Thetypeof luciferase(Renilla. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. Promega. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. SpectraMax M5. . The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. Use the 3. . For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Additionally, user-installable options allow the SpectraMax i3 System to. . 1321N1, a human astrocytoma cell line. SpectraMax i3x Multi-Mode Microplate Reader. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). 1. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. It allows you to explore cellular . Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Facilities. 2. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax.